Dhruv Ripudeman Singh Bhadoriya

Integrated normalized liver cancer transcriptomics data with Human-GEM model using COBRA Toolbox in MATLAB. Executed Differential Flux Analysis to decipher altered metabolic pathways and constructed a Drug-Gene Interaction Network for potential therapeutics.

Investigated stress-induced mitochondrial metabolic adaptations using a yeast mitochondrial genome-scale model. Mapped differential gene expression of yeast cells to mitochondrial metabolic fluxes in MATLAB and implemented integration algorithms to refine flux distributions.

Demonstrated and supervised experimental techniques including protein quantification and enzyme kinetics assays. Provided hands-on training in pipetting, reagent preparation, lab safety, experimental design, and data interpretation.

Assisted in preparing lab protocols, demonstrating experiments, and teaching basic working principles of biosensors. Supported students in using biosensor devices, including sensor calibration and data interpretation during lab sessions.

Analyzed the impact of mitochondrial respiration on the fluidity of the inner membrane of isolated mitochondria. Isolated GFP-tagged mitochondria from S. cerevisiae and assessed fluidity using Fluorescence Lifetime Imaging Microscopy.

Investigated metabolic alterations across healthy and NAFLD-affected liver tissues using differential flux analysis. Pre-processed and TPM-normalized transcriptomics data with R, reconstructed context-specific models in MATLAB, and performed analysis using CobraPy in Python.

Engineered E. coli for DNA transfer to mammalian mitochondria using antigen-nanobody binding. Executed nanobody expression via plasmid cloning and achieved antigen expression on HEK293 cells, verifying transfer via PCR-based detection.

Determined differentially expressed genes in metabolic disorders and their associations with circadian rhythm. Performed differential gene expression analysis on bulk cell RNA-Seq datasets using Limma in R and visualized data with Volcano Plot and Heatmap.

Developed a user-friendly toolbox for predictive analysis of mutation consequences of amino acid sequences. Designed a 'Mutation Tool' interface using HTML, CSS, and JavaScript, integrating mutation capabilities with an embedded library.

Grade: 9.23

Activities and societies: National Service Scheme (NSS), National Cultural Appreciation - Dance (NCA), Spectra (Fine Arts Club)

Final-year undergraduate at IIT Kharagpur, majoring in Biotechnology and Biochemical Engineering, led research in Systems and Synthetic Biology, Molecular Biology, and Computational Biology.