Passong Immanual Chhakchhuak

Built and standardized an end-to-end mRNA production workflow, achieving ~95% purity and supporting up to 30 mg per run. Led recombinant protein and antibody production, established in-house GPC3 antigen and minibinder production, and developed SOPs while training junior scientists.

Investigated molecular mechanisms controlling bacterial transcription, developing biochemical assays and identifying regulatory regions. Mentored junior researchers and contributed to published research and standardized workflows.

Helped establish a molecular microbiology laboratory from the ground up, handling equipment selection, procurement, and vendor coordination. Served as the experimental biology resource, conducting comparative bacterial transcription studies and collaborating on transcriptomic analysis and machine learning.

Developed deep expertise in bacterial transcription regulation, establishing genome-wide understanding of termination control. Developed computational frameworks and biochemical assays, including a screen of ~30,000 bacterial colonies, and engineered strains to investigate mechanisms.

Pursued a Ph.D. in Biotechnology, focusing on bacterial transcription regulation and developing genome-wide understanding of transcription termination.

Completed a Master of Science in Biotechnology, building foundational knowledge in biotechnological methods and research.

Earned a Bachelor of Science in Biotechnology, gaining a broad understanding of biological sciences and laboratory techniques.