Gonzalo Miranda

• Investigate how early-life resource scarcity affects adult cortical vascular morphology using the LBN model, immunohistochemistry, confocal microscopy, and Imaris 3D reconstruction of somatosensory vasculature.
• Develop image-analysis and neuroimaging workflows across preclinical diffusion MRI and human fMRI datasets, including DSI Studio tractography, FA/MD/AD/RD feature extraction, MATLAB fun

• Supported R&D workflows for Cultrex Basement Membrane Extract production and iPSC-related applications for cell and gene therapy research.
• Redesigned production workflows and reduced batch failure rates from 54% to 0% over 14 months while minimizing hazardous waste.
• Helped resolve a 7-month methylcellulose backorder for ELISA systems as part of a national task force, supporting recovery from

Coordinated 6 weekly classes for 3 topics over 4.5 hrs of instruction for 170+ students. Refined and audited general curricula for Advanced Middle and High school honors track. Implemented IEP’s for 25+ students as directed. Monitored Covid distance learning: mapped student metrics timeline, maintained weekly standards compliance, student IEP’s, and reviews.
• Maintained a passing average above 86

Project: Inter-Neuronal Gama Oscillation Modelling of Fear Conditioning in the Amygdala
Investigated neurological pathway mapping of fear conditioning in the amygdala based on inter-neural Gamma oscillation analysis. Analyzed data from mice via neuroimaging and amygdala slice culture spectrogram for use in mapping out fear signature conditioning pathway(s) through the feedback network regions.

Performed regular laboratory maintenance, inventory management, lab material/reagent sterilization, and hazardous emergency procedures. Maintained documentation of lab quality control, incident reports, data verification, and protocol compliance
• Collected, prepared, cleaned/sterilized, ordered, stored, and set up lab/research supplies and equipment as directed
• Served as Chem Dept. liaison whil

Project: Analysis of Post Impact Trauma Tissue Recovery via Screening of 18S, IL1-B, and IL6 Biomarkers
Assessed effects of blunt force trauma on cell recovery of simulated cortical tissue via 18S, IL1-B, and IL6 biomarker tracked regeneration of endothelial cell culture on lyophilized silk scaffolds or collagen hydrogels
• Fabricated scaffolds & hydrogels, seeded cell tissues, and ensured cell c

Investigated and confirmed an increase in biocompatibility of silk cornea model by matching silk degradation rate to seeded cell proliferation rate. Quantified and manipulated degradation rate variations of cornea modeled silk scaffolds by controlling protein β-sheet content and %wt of silk fibroin solution via water annealing.
• Processed Bombyx Mori silk to extract fibroin, formed layered patter

• Design Lesson Plans and Workshops for student between grades 5th to 8th based on STEM; focusing on 3D modelling AutoCAD, and programming in RobotC.
• Train incoming instructors in curriculum and activity execution
• Hold daily performance meetings with teaching staff & group leaders to examine possible issues/improvements