Sharvari Pathak
@sharvaripathak
Graduate biomedical research student advancing cardiac bioprinting and regenerative medicine with data-driven AI learning.
What I'm looking for
I’m a Graduate research student at the Wake Forest Institute of Regenerative Medicine (WFIRM), where I focus on functional cardiac patch bioprinting. I cultivate and differentiate human iPS cells into cardiomyocytes, synthesize cardiac-specific bioink, and incorporate both into a 3D muscle model to build more functional cardiac tissue.
Previously, I worked in molecular and immunological research at Columbia University Irving Medical Center, conducting ELISA, ELISpot, and flow cytometry, along with genomic DNA preparation for genotyping and expression studies using Northern analysis, Western blotting, and quantitative PCR. Earlier experience includes producing high-brightness nanoparticle-based dyes for flow cytometry detection, performing in-situ hybridization and fluorescent microscopy in HeLa cells, and supporting dye/material characterization, purification, and antibody conjugation. I also strengthen others through teaching and tutoring in Principles of Biology, emphasizing lab safety, study strategy, and interactive learning.
Experience
Work history, roles, and key accomplishments
Graduate Research Student
Wake Forest Institute of Regenerative Medicine (WFIRM)
Sep 2024 - Present (1 year 6 months)
Conducts functional cardiac patch bioprinting by culturing and differentiating human iPS cells into cardiomyocytes. Synthesizes and applies cardiac-specific bioinks and integrates bioink and cells into a 3D muscle model.
Reinforced learning in an AI model on complex regenerative medicine topics by designing domain-specific prompts. Evaluated and revised model responses, including inducing reasoning errors to strengthen learning.
Laboratory Technician
Columbia University Irving Medical Center
May 2022 - May 2024 (2 years)
Performed molecular, genetic, and immunological experiments to study the genetic basis of kidney disease in human and murine models. Conducted ELISA/ELISpot and flow cytometry, and performed DNA preparation plus gene/protein expression assays including Northern blot, Western blot, and qPCR.
Research Technician
StabiLux Biosciences
Jan 2022 - Apr 2022 (3 months)
Produced high-brightness dye materials using nanoparticles for cell detection in flow cytometry. Conducted in-situ hybridization and fluorescent microscopy, and supported dye/antibody synthesis, characterization, and purification for conjugation workflows.
Research Assistant (Metastasis)
University of Michigan–Flint
Aug 2021 - Dec 2021 (4 months)
Investigated metastasis behavior of breast cancer cell lines under stress conditions using protein analysis and 3D invasion models. Performed Western blotting (including Furin detection) and supported mammalian cell culture for MB-468 and MCF-7.
Research Assistant (Antioxidants)
University of Michigan–Flint
Jan 2021 - May 2021 (4 months)
Evaluated solvent systems for analysis of antioxidants to identify less hazardous and harmful options. Conducted electrochemical experiments, analyzed analytical data, and prepared antioxidant standards for testing.
Education
Degrees, certifications, and relevant coursework
Wake Forest University
Master of Science, Biomedical Research
2024 - 2026
Pursuing an MS in Biomedical Research at Wake Forest University from 2024 to 2026.
University of Michigan, Flint
Bachelor of Science, Molecular Biology and Biotechnology
2018 - 2021
Grade: GPA: 3.84/4.0
Activities and societies: College Dean’s List (2018–2021). Recipient of Maize and Blue Scholarship (2021) and Brainstorm Freshman Scholar Award (2019).
Earned a BS in Molecular Biology and Biotechnology (research-focused concentration) at the University of Michigan, Flint from 2018 to 2021.
Tech stack
Software and tools used professionally
Availability
Location
Authorized to work in
Job categories
Skills
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