Muhammad Hussain

I've led CRISPR-based editing of glucosinolate biosynthesis genes in Brassica crops at Sejong University, advancing a pipeline toward improved nutritional traits. I also generated molecular and physiological data on GABA metabolism in tomato under salinity and osmotic stress to inform stress-tolerance breeding.

During my PhD, I built CRISPR/Cas9 knockout lines targeting GABA-T3 in tomato, confirming mutations and connecting the gene to stress-response and reproductive phenotypes.

I engineered MYB28 transcription factor variants that increased glucoraphanin accumulation in Brassica crops. I also developed and co-invented a patented generalized protoplast regeneration protocol for broccoli, cabbage, cauliflower, and lettuce, supporting future DNA-free genome editing applications.

Across four or more concurrent projects, I've managed cloning, plant transformation, expression analysis, and metabolite quantification from gene target through validated edited lines. I'm a co-inventor on a Korean patent application and author of five peer-reviewed publications.