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Yesasvi Nandigam

@yesasvinandigam

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Bioinformatics analyst specializing in reproducible NGS and metagenomic pipelines with statistical modeling.

United States

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What I'm looking for

I’m looking for a bioinformatics role where I can build reproducible NGS pipelines, apply rigorous statistical analysis, and collaborate closely to turn complex multi-omic and metagenomic data into clear biological insights.

I’m a Bioinformatics Analyst applying reproducible NGS and metagenomic workflows to real biological questions. At Karyosoft Inc., I analyzed 300+ rice phyllosphere metagenomic samples using Bowtie2, Kraken2, and Bracken, and used CLR-transformed genus profiles with PERMANOVA to identify 3 microbial community clusters (p=0.001, ARI=0.79).

I also build robust, end-to-end pipelines—most recently a genotype imputation pipeline with BEAGLE 5.4 on HPC, processing 2.67M SNPs across 5 Arabidopsis chromosomes. I found MAF as the strongest predictor of imputation accuracy, achieving r²=0.913 at MAF 20–50%, and I deploy components on AWS using Docker for reproducible execution.

Earlier, as a Research Assistant at the Luddy School of Informatics and Computing, I built multi-omic seq pipelines integrating HISAT2/STAR alignment, GATK variant calling, QC with FastQC/fastp/MultiQC, and differential expression with DESeq2/edgeR. I’ve complemented this with teaching and communication—serving as a Teaching Assistant (R and Python) and delivering 15+ literature review presentations—while my projects include WNN multimodal single-cell immune heterogeneity work and a Snakemake-based variant calling benchmark where GATK HaplotypeCaller achieved 97.34% F1.

Experience

Work history, roles, and key accomplishments

Current

Bioinformatics Analyst

Current

Karyosoft Inc

Aug 2025 - Present (11 months)

Analyzes rice phyllosphere metagenomic samples to profile bacterial community composition and cluster ecological differences. Builds and deploys an end-to-end genotype imputation pipeline on HPC (BEAGLE) and AWS using Docker.

Kraken2 Bracken CLR Transformation PERMANOVA Shannon Index Simpson Index Kruskal Wallis Test BEAGLE 5.4 HPC AWS Docker NCBI

Research Assistant

Luddy School of Informatics and Computing

Sep 2023 - May 2025 (1 year 8 months)

Builds multi-omic NGS pipelines integrating alignment, QC, variant calling, and differential expression across samples. Automates workflows on Linux HPC with Slurm and supports research through analyses, visualizations, and teaching assistant duties.

HISAT2 Star GATK MultiQC DESeq2 Python R HPC Slurm Heatmaps Volcano Plots Ggplot2

Research Intern

Dr. Reddy Laboratories

Jan 2023 - Jun 2023 (5 months)

Supports GMP-compliant sterile and environmental monitoring work by performing aseptic techniques and documenting microbial growth outcomes. Assists with SOP preparation/validation and maintains batch records for data traceability.

GMP Aseptic Technique Environmental Monitoring Microbial Growth Documentation Batch SOP Development Quality Control

Education

Degrees, certifications, and relevant coursework

Indiana University Indianapolis

Master of Science in Bioinformatics, Bioinformatics

2023 - 2025

Grade: GPA: 3.6/4.0

Activities and societies: Coursework: Applied Statistics; NGS Data Analysis; Cloud Computing; Machine Learning; Systems Biology; Algorithms and Data Structures.

Master of Science in Bioinformatics (2023–2025) with coursework in applied statistics, NGS data analysis, cloud computing, machine learning, systems biology, and core algorithms/data structures.

MNR Degree College

Bachelor of Science in Genetics, Genetics

2019 - 2022

Grade: GPA: 3.3/4.0

Activities and societies: Coursework: Human Genetics; Molecular Biology; Fundamentals of Bioinformatics; Microbiology; Chemistry.

Bachelor of Science in Genetics (2019–2022) including coursework in human genetics, molecular biology, fundamentals of bioinformatics, microbiology, and chemistry.