
Mohammad Elsharkawy
@mohammadelsharkawy
Bioinformatics and computational biology researcher building SeqQC, an R package for reproducible FASTA quality control.
What I'm looking for
I built SeqQC, an R package that automates FASTA quality checks for GC content, N-content, and sequence validity, and produces reproducible reports. I also created a Python DNA Toolkit for sequence parsing, validation, and exploratory analysis.
As a Research Assistant at Faculty of Medicine, University of Jordan, I record calcium imaging of cultured DRG neurons and quantify neuronal activity through image analysis. I also score behavioral videos, analyze datasets, and contribute to data interpretation and manuscript preparation.
My laboratory work has included DNA extraction, PCR, genotyping, MLPA, and cytogenetic analysis. I’ve co-authored two peer-reviewed papers, and my wet-lab experience helps me understand where biological data come from.
Experience
Work history, roles, and key accomplishments
Research Assistant
University of Jordan
Aug 2025 - Present (1 year 2 months)
Record calcium imaging of cultured DRG neurons and quantify neuronal activity by image analysis. Score behavioral videos and analyze datasets; contribute to data interpretation and manuscript preparation.
Laboratory Technician
National Center for Diabetes, Endocrinology and Genetics
Aug 2025 - Sep 2025 (1 month)
Performed automated DNA extraction, MLPA, reverse hybridization and genotyping on clinical samples.
Trainee Cytogeneticist
National Center for Diabetes, Endocrinology and Genetics
Jul 2025 - Aug 2025 (1 month)
Performed karyotype analysis and aneuploidy detection on peripheral blood, amniotic fluid and chorionic villus (CVS) samples.
Research Assistant
Jordan University of Science and Technology
Mar 2025 - Jul 2025 (4 months)
Analyzed outcomes with ANOVA and post-hoc tests in Minitab.
Laboratory Technician
Jordan University of Science and Technology
Feb 2023 - Feb 2025 (2 years)
Supported molecular and cytogenetic lab operations for two years: sample handling, cell culture, PCR, gel electrophoresis and routine quality procedures.
Laboratory Technician
Royal Hospital
Sep 2024 - Nov 2024 (2 months)
Performed CBC, ESR, PT/PTT, clinical chemistry panels, blood smears, urinalysis, stool analysis and antimicrobial susceptibility testing.
Education
Degrees, certifications, and relevant coursework
Harvard University
CS50P Python and CS50R, Computer Science
Completed CS50P Python and CS50R courses through edX.
University of Leeds
Introduction to Programming Using Python, Computer Science
Completed Introduction to Programming Using Python course.
MathWorks
MATLAB Onramp, Signal and Image Processing Onramps, Engineering
Completed MATLAB, Signal and Image Processing Onramps.
GenQA GENie
Clinical Genetics Training Modules, Clinical Genetics
Completed clinical genetics training modules including HGVS Nomenclature, ISCN Constitutional Karyotyping, Constitutional FISH, Neoplasia Karyotyping, Risk Calculations, and BRCA1/BRCA2/HRR variant classification.
QIAGEN
Digital PCR Course, Laboratory Methods
Completed Digital PCR course with CPD accreditation.
Jordan University of Science and Technology
Bachelor of Science, Biotechnology and Genetic Engineering
2021 - 2025
Grade: 4.03/4.00
Activities and societies: Relevant coursework: Bioinformatics, Elements of Biostatistics, Programming in C++, Human Genetics, Molecular Genetics.
B.Sc. in Biotechnology and Genetic Engineering with a GPA of 4.03/4.00, distinguished with honour list in 7 of 8 semesters. Research project received an A+.
Availability
Location
Authorized to work in
Portfolio
github.com/PredVI/DNA-ToolkitSalary expectations
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