At University Hospital Magdeburg, I isolated primary cholangiocytes from mouse liver and established a long-term 3D organoid culture. I optimised the matrix, medium and passaging strategy, and developed and validated ELISA, RT-qPCR, Western blot and immunofluorescence readouts for the model.
I used this platform to study bile acid receptor signalling in genetically modified mouse lines, including TGR5/GPBAR1. I planned experiments, analysed results statistically and presented findings, including a first-author oral presentation at UEG Week 2025.
I wrote the SOPs and work instructions used in my lab, maintained traceable records and trained three colleagues hands-on. I’m completing my PhD at Otto von Guericke University / University Hospital Magdeburg, with my dissertation defence expected in March 2027.
