Dhairya Pancholi
@dhairyapancholi
I am an experienced scientist specializing in immunoassays and GMP-compliant testing
CanadaWhat I'm looking for
I lead a small laboratory team performing antibiotic potency and microbiological testing under GMP/GLP compliance, focusing on method validation, SOP development, and client-facing result interpretation. I prioritize data integrity, ALCOA+ principles, and audit readiness in regulated lab environments.
My research background includes engineering gene constructs, generating stably transfected cell lines, and using proximity-dependent biotinylation (BioID) to map protein interactomes. I have hands-on expertise in western blotting, high-resolution immunofluorescence microscopy, and foundational ELISA workflows, supported by peer-reviewed publications.
I bring strong assay development, troubleshooting, and scientific communication skills, with experience in environmental monitoring, eLIMS data management, OOS investigation, and CAPA documentation. I am passionate about advancing diagnostic innovation through robust immunoassay design and translational research.
Experience
Work history, roles, and key accomplishments
ETLead a team of three analysts conducting antibiotic potency testing (cylinder plate and turbidimetric) per USP/EP; optimized assay protocols, managed eLIMS sample tracking, and maintained GLP/GMP compliance to support timely client deliveries.
Thesis Research
Toronto Metropolitan University
Sep 2019 - Aug 2023 (3 years 11 months)
Developed protein interactomes for ADPKD and Batten disease using BioID; engineered five gene constructs, generated 11 stable cell lines, and validated assays (Western blot, immunofluorescence) under GLP-aligned SOPs.
Research Assistant
Institute of Human Genetics
Apr 2016 - Oct 2018 (2 years 6 months)
Analyzed mutational data from 1,000 patient DNA samples using PCR-RFLP to determine carrier frequency for Gaucher disease and led ARMS-PCR screening of 200 samples, contributing to peer-reviewed publications.
Thesis Research
Symbiosis International University
Jun 2013 - Feb 2016 (2 years 8 months)
Investigated quercetin effects on SK-MEL-28 cells using MTT, wound-healing, invasion assays, flow cytometry, and qPCR to determine IC50 (50 μM) and demonstrate reversal of EMT markers.
Education
Degrees, certifications, and relevant coursework
TUToronto Metropolitan University
Master of Science, Molecular Science
2019 - 2023
Grade: Governor General Gold Medal nominee
Activities and societies: Thesis: Developing Protein Interactome For Rare Genetic Diseases Using BioID (ADPKD and Batten disease); maintained GLP-compliant cell culture, standardized SOPs, and performed Western blot and immunofluorescence validation.
Completed M.Sc. in Molecular Science with a thesis developing protein interactomes for rare genetic diseases using BioID, generating stable cell lines and validating protein-protein interactions.
SUSymbiosis International University
Master of Science, Biotechnology
2013 - 2015
Activities and societies: Thesis: Inhibitory Effect Of Quercetin On Epithelial To Mesenchymal Transition in SK-MEL-28 human melanoma cells; performed MTT assays, wound healing, invasion assays, qPCR, and flow cytometry.
Earned M.Sc. Biotechnology with a thesis investigating quercetin's inhibitory effects on epithelial-to-mesenchymal transition in SK-MEL-28 cells, determining IC50 and demonstrating antiproliferative and apoptotic effects.
GUGujarat University
Bachelor of Science, Biotechnology
2009 - 2012
Completed B.Sc. in Biotechnology with coursework and laboratory training in molecular biology, cell culture, and biochemical techniques.
Tech stack
Software and tools used professionally
Availability
Location
CanadaAuthorized to work in
Job categories
Skills
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